Abstract
Objective To develop an RP- HPLC method for determination of tanshinol,protocatechualdehyde, paeoniflorin,puerarin,ferulic acid,tanshinoneⅡA and astragaloside in blood-invigorating and stasis-removing prescription(BSP).Methods The analysis was performed with a column of Waters Symmetry ShieldTM RP C18(150mm × 4.6mm,3.5 μm),and the mobile phase consisted of methanol-0.25% acetic acid.The flow rate was 0.8 mL/min and the column temperature was 30℃.UV was employed to determine the contents of tanshinol,protocatechualdehyde,paeoniflorin,puerarin, ferulic acid and tanshinoneⅡA,and the detection wavelength was set at 280nm.Evaporative light scattering detection(ELSD) was employed to determine the contents of astragaloside.The temperature of drift tube was 90℃ and the gas flow was 2.8L/min(compressed air).Results The linearity was obtained over 0.01-0.80μg(r=0.999 8)for tanshinol,0.005-0.4 μg(r=0.999 7)for protocatechualdehyde,0.05-4μg(r=0.999 8)for paeoniflorin,0.005-0.4μg(r=0.999 7)for puerarin,0.006-0.5μg(r=1)for ferulic acid,0.005-0.4μg(r=1)for tanshinoneⅡA,and 0.031-2.46μg(r=0.999 3)for astragaloside.The recoveries were all between 97.0%-101.0%,and RSDs were all less than 2%.The contents(mean) of tanshinol,protocatechualdehyde,paeoniflorin,puerarin,ferulic acid,tanshinoneⅡA and astragaloside in three batches of samples were 1.15,0.13,4.48,0.80,0.72,0.31and 3.12mg/g,respectively.Conclusion The method in our study is convenient,accurate and sensitive,and it provides a reference for the determination of active ingredients in BSP.
Published Version
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