Abstract
Starch is widely distributed in diverse plant organs as a reserve carbohydrate; it is also a major source of carbohydrates in human food. Because of its importance, different methods have been developed to measure the starch content of food and feed. Planar chromatography has been used to measure starch content of cereal products. The starch was hydrolyzed using α-amylase and amyloglucosidase and the resulting glucose was separated on silica gel 60 HPTLC plates and quantified at λ = 520 nm after derivatization. The glucose calibration plot was linear between 100 and 300 ng per spot with a coefficient of determination ( r 2 ) of 0.9959. The limits of detection (LOD) and quantification (LOQ) for starch as glucose were 0.26 and 0.51 (g per 100 g), respectively. The mean concentrations of starch in wheat flour and in starch premix (an industrial product) were 74.56 ± 2.58% and 84.85 ± 1.96%, respectively. The proposed method was shown to be a precise, selective, and sensitive means of measuring starch in cere...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.