Abstract
The recovery of simian rotavirus SAl1 from dechlorinated 10l tap water samples shows a marked improvement when ultrafiltration or hydroextraction are used as second-step concentration methods. After initial adsorption of virus on to a nitrocellulose filter and subsequent elution with 3% beef broth, organic flocculation gave an average recovery of 4%. Ultrafiltration gave an average recovery of 48% while hydroextraction in crystalline sucrose gave a recovery rate of 60%. Rotavirus recovery rates were quantified by the immunofluorescence assay.
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