Abstract
To determine whether rosiglitazone-enriched diet offer protection in a classical model of liver ischemia-reperfusion injury in rats. Two days before the experiment, rats were divided into 2 groups: Control Group (n=13) rats fed with standard diet; Rosi Group (n=13): rats fed with a powdered standard diet supplemented with rosiglitazone. The animals were submitted to liver ischemia-reperfusion by clamping the pedicle of median and left anterolateral lobes. After 1 hour of partial hepatic ischemia, the clamp was removed for reperfusion. After 2 or 24 hours (Control and Rosi Groups), blood was collected for enzymes and cytokines analysis. Ischemic and non-ischemic liver were collected for malondialdehyde analysis and histological assessment. Lungs were removed for tissue myeloperoxidase quantification. There were no statistical differences between groups for all analysed parameters. In this model, rosiglitazone-enriched diet did not protect liver against ischemia-reperfusion injury.
Highlights
Liver ischemia-reperfusion injury (LIRI) is a phenomenon inevitable after hepatic surgery, liver transplantation, shock and trauma[1]
One speculate if peroxisome proliferator-activated receptors (PPARs)--Ȗ agonists may have a therapeutic role in liver ischemia-reperfusion injury
Our aim was to determine whether rosiglitazone-enriched diet could offer protection in a classical model of liver ischemia-reperfusion injury in rats
Summary
Liver ischemia-reperfusion injury (LIRI) is a phenomenon inevitable after hepatic surgery, liver transplantation, shock and trauma[1]. Thiazolidinediones (pioglitazone, troglitazone, and rosiglitazone) are synthetic PPAR--Ȗ agonists and they act to enhance insulin sensitivity and reduce serum glucose in diabetic patients[5]. They are widely used as antidiabetic agents. A recent study showed that PPAR--Ȗ plays an inhibitory role in LIRI and the stimulation by selective agonist has a significant beneficial effect in mice. This protective effect of pioglitazone was associated with downregulation of the local expression of several potent proinflammatory cytokines, chemokines and adhesion molecules after reperfusion[7]. Our aim was to determine whether rosiglitazone-enriched diet could offer protection in a classical model of liver ischemia-reperfusion injury in rats
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