Rosiglitazone protects acute kidney injury in septic rats

Abstract

To investigate the effects of rosiglitazone on the structure of the kidney of rats in sepsis with acute kidney injury. Sepsis models were established using male Sprague-Dawley rats by cecal ligation puncture (CLP). 96 healthy rats were randomly divided into 4 groups: cecal ligation puncture and rosiglitazone group (group CLP+ ROSI), cecal ligation puncture group (group CLP), Sham and rosiglitazone group (group Sham+ ROSI), sham group (group Sham). Six rats were sacrificed at 0, 6, 12, 24 h after CLP respectively in each group. The general status of rats was observed and H&E staining was applied to detect renal pathological histology changes. Myeloperoxidase(MPO) was measured in kidney tissues. Expression of the ligand of peroxisome proliferators-activated receptor γ (PPARγ), and B-cell lymphoma-2(Bcl-2) were also determined by Western blot analysis. The increased MPO activity of group CLP+ ROSI was significantly lower than that of group CLP at 12 h and 24 h[(4.07±0.16)vs(4.62±0.08); P12=0.01, P24=0.006]. The MPO activity of group CLP was significantly higher than that of group Sham at 6, 12 and 24 h[(4.62±0.08)vs(1.30±0.06); P6=0.00, P12=0.00, P24=0.00]. The MPO activity of group CLP+ ROSI was significantly higher than that of group Sham+ ROSI at 6, 12 and 24 h[(4.07±0.16)vs(1.27±0.06); P6=0.00, P12=0.00, P24=0.00]. Compared with group Sham and group Sham+ ROSI, in rats of group CLP and group CLP+ ROSI the symptoms of sepsis and organ dysfunction were observed. The symptoms of sepsis and organ dysfunction of group CLP+ ROSI were significantly reduced than that of group CLP. Expression of PPARγ and Bcl-2 were determined by Western blot analysis, the expression of PPARγ and Bcl-2 of group CLP+ ROSI was significantly lower than that of group CLP. Rosiglitazone, as the ligand of PPARγ, reduced the inflammatory response in sepsis, decreased the apoptosis of kidney cell, reduced kidney injury, and protected acute kidney injury in rats of sepsis.