Effects of rosiglitazone on levels of TNFα in septic rats with acute kidney injury

Abstract

To investigate the influence of rosiglitazone on tumor necrosis factor-α (TNFα) of rats in sepsis with acute kidney injury. Sepsis models were established using male Sprague-Dawley rats by cecal ligation puncture (CLP). Ninety six healthy rats were randomly divided into 4 groups: cecal ligation puncture and rosiglitazone group (group CLP+ROSI), cecal ligation puncture group (group CLP), Sham and rosiglitazone group (group Sham+ROSI), Sham group (group Sham). Blood samples of 6 rats in each group were collected at postoperative 0, 6, 12, 24 h, to detect TNFα levels in serum. Observe and compare the mobility of rats in each group and the level of serum creatinine and H&E staining was applied to detect renal pathological histology changes. Statistics was analyzed by SPSS 13.0 software application of independent samples t test. The creatinine level of Group CLP was increased with the extension of time, the creatinine level of group CLP was significantly higher than that of group Sham at 12 h and 24 h (P(12) = 0.047, P(24) = 0.001). The creatinine level of group CLP+ROSI was significantly higher than that of group Sham+ROSI at 12 h and 24 h (P(12) = 0.033, P(24) = 0.039). The increased creatinine level of group CLP +ROSI was significantly lower than that of group CLP at 12 h and 24 h (P(12) = 0.04, P(24) = 0.006). The TNFα concentration of group CLP was significantly higher than that of group Sham at 6 h, 12 h and 24 h (P < 0.05). The TNFα concentration of group CLP+ROSI was significantly higher than that of group Sham+ROSI at 6 h, 12 h and 24 h (P < 0.05). The increased TNFα concentration of group CLP+ROSI was significantly lower than that of group CLP at 6 h, 12 h and 24 h (P(6) = 0.01, P12 = 0.02, P(24) = 0.01). Compared with group Sham and group Sham+ROSI, in rats of group CLP and group CLP+ROSI the symptoms of sepsis and organ dysfunction were observed. The symptoms of sepsis and organ dysfunction of group CLP+ROSI were significantly reduced than that of group CLP. Rosiglitazone can inhibit the expression of TNFα in serum of septic rats, reduce the inflammatory reaction, reduce kidney injury, improve the renal function. Rosiglitazone on septic rats with acute kidney injury has a protective effect.