Abstract
This paper discusses a method of micropropagation of Vaccinium corymbosum L. cv. Blue-Berry. The results showed that WPM supplemented with 1,0 mg/l of zeatin in combination with 0,1 mg/l of indolyl-3-butyric acid was more effective for the multiplication of blueberry axillary shoots. The maximum increase in the number of healthy axillary shoots was observed in the fourth subculture, whereas the phenomenon of hyperhydration (vitrification) began to appear in the fifth subculture. In addition, it was established that the presence of indolyl-3-butyric acid and 1,0 g/l of activated charcoal in the nutrient medium lead to the development of good root system of the Vaccinium corymbosum cv. Blue-Berry. The highest mean number of roots formed per explant was obtained on WPM medium, supplemented with 0,5 mg/l indolyl-3-butyric acid after 10 weeks. Acclimatization of in vitro regenerated plantlets of Vaccinium corymbosum with a developed root system in ex-vitro conditions (pH 3,54) showed a 100% survival rate.
Highlights
Мохамед Гамил Райян Абуэлдис, аспирант кафедры ботаники и физиологии растений Хуснетдинова Ландыш Завдетовна, кандидат биологических наук, доцент кафедры ботаники и физиологии растений Тимофеева Ольга Арнольдовна, доктор биологических наук, профессор кафедры ботаники и физиологии растений
The paper describes the study of steppe vegetation in the Samara Region
Geobotanical relevès were made on standard sites for steppe vegetation
Summary
Мохамед Гамил Райян Абуэлдис, аспирант кафедры ботаники и физиологии растений Хуснетдинова Ландыш Завдетовна, кандидат биологических наук, доцент кафедры ботаники и физиологии растений Тимофеева Ольга Арнольдовна, доктор биологических наук, профессор кафедры ботаники и физиологии растений. It contains results of the steppe communities field study conducted in 2014–2015 in the vicinity of Togliatti (Samara Region). Что микроразмножение пазушных побегов голубики высокорослой было более эффективным на WPM с добавлением 1,0 мг/л зеатина и 0,1 мг/л индолил-3-масляной кислоты. Акклиматизация растений-регенерантов V. corymbosum, имеющих развитую корневую систему, при переносе в естественные условия ex vitro (рН 3,5–4) показала 100% выживаемость.
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