Abstract

A new typical and high efficient rooting and transplanting method of Populus davidiana × P. bolleana was set up from hardening off seedlings,moisturizing and pollution prevention in the study. Firstly,put the tissue culture P. davidiana × P. bolleana seedlings in large test tubes with liquid medium for rooting for 12- 15 d. Secondly,refine hydroponic seedlings in the aseptic water for 2 d. Then,transplant the seedlings into soil and cover with plastic cups to moisturizing,which will be removed after 15 d. At last,the 17 d old seedlings with soil were transplanted into the field. Using the above method,the survival rate of transplanting seedlings can increase to more than 98%. The advantage of the method is not only economic,simple,small-scaled and high efficient but also suitable for many other woody plants,so it is easy to generalize.

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