Abstract
In order to improve the rooting rate of Qiuzi pear tissue culture seedlings, this study took 'sorb' as experimental material, based on Qiuzi pear rooting tissue culture medium (1/2MS+IBA 0.5 mg/L), and added different concentrations of activated carbon (AC): 0.25 g/L, 0.50 g/L, 0.75 g/L, 1.00 g/L. The results showed that the rooting rate, rooting number and root length of tissue culture seedlings cultured with activated carbon were significantly higher than those of the control group. The most suitable medium for rooting Qiuzi pear was 1/2MS+IBA 0.5 mg/L+AC 1.00 g/L, and the rooting rate of the treated sorb tissue culture seedlings reached 90%, which could obviously promote the growth of Qiuzi pear tissue culture. The results provide a theoretical basis for improving the rooting rate and survival rate of tissue culture seedlings of pear plants, and provide a reference for the establishment of rapid propagation system of woody plants.
Highlights
Autumn pear (Pyrus ussuriensis Maxim.) is one of the important pear species in China, which is abundant in Northeast China, Hebei and Shandong
The results showed that the rooting rate, rooting number and root length of tissue culture seedlings cultured with activated carbon were significantly higher than those of the control group
1.1 Effects of different activated carbon (AC) concentrations on the growth of tissue culture seedlings of ‘sorb’ On the 14th day, red young roots appeared in the roots of ‘sorb’ tissue culture seedlings
Summary
Autumn pear (Pyrus ussuriensis Maxim.) is one of the important pear species in China, which is abundant in Northeast China, Hebei and Shandong. Abstract In order to improve the rooting rate of Qiuzi pear tissue culture seedlings, this study took ‘sorb’ as experimental material, based on Qiuzi pear rooting tissue culture medium (1/2MS+IBA 0.5 mg/L), and added different concentrations of activated carbon (AC): 0.25 g/L, 0.50 g/L, 0.75 g/L, 1.00 g/L. The results showed that the rooting rate, rooting number and root length of tissue culture seedlings cultured with activated carbon were significantly higher than those of the control group.
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