Root morphology, nitrogen metabolism and amino acid metabolism in soybean under low phosphorus stress.

Abstract

Phosphorus deficiency is a major influence on growth and development of soybean. Therefore, improving phosphorus utilization efficiency in soybean is a research priority for the soybean community. In this experiment, Liaodou 13 (high phosphorus utilization: HPE) and Tiefeng 3 (low phosphorus utilization: LPE) were used as test varieties. We investigated changes in root morphology, amino acid content, and content of key substances of the nitrogen metabolic pathway with normal phosphorus (0.5 mM) and low phosphorus (0.005 mM) treatments. The results showed that the root length, root surface area and number of lateral roots of HPE roots were higher than those of LPE roots under normal and low phosphorus conditions. The contents of different types of amino acids showed different trends in two varieties. The HPE showed small change in the content of total hydrolyzed amino acids under the low phosphorus condition when compared to the normal phosphorus treatment by a 6.67% decrease, on the contrary LPE showed a drastic decrease by 20.36%. However, HPE exhibited similar decreasing trends in the contents of hydrolyzed and free aspartic acid with the low phosphorus treatment. Moreover, the contents of free histidine and valine in LPE were significantly increased by 657.84% and 149.29% respectively, in contrast to significant decreases in HPE. In aspects of major nutrient elements, the contents of phosphorus, total nitrogen and ammonia nitrogen in both HPE and LPE varieties decreased to dramatic levels. However, the nitrate nitrogen content significantly increased 78.51% for HPE and 65.12% for LPE. Compare to the normal condition, the GOGAT activity in HPE decreased by 5.18% but increased by 33.10% in LPE. Compare to the normal condition, the GS activity in HPE increased by 7.26% but decreased by 21.72% in LPE under phosphorus deficiency. In summary, the phosphorus-efficient soybean variety HPE exhibited superior tolerance to low phosphorus deficiency through advantageous root morphology, phosphorus uptake and transfer capability, and balanced amino acid metabolism and nitrogen metabolism pathways.