Root extracts of Polygala tenuifolia for the green synthesis of gold nanoparticles.

Abstract

Traditional medicinal plants possess diverse active constituents for exerting their biological activities. Recently, the innovative applications of plant extracts have revealed their promise as 'green' reducing agents for the reduction of metal ions during the synthesis of metallic nanoparticles. Herein, we report the use of 70% ethanol extracts from Polygala tenuifolia roots as a 'green' reducing agent for the production of gold nanoparticles by reducing gold(III) chloride trihydrate. Gold nanoparticles were characterized using UV-Visible spectrophotometry, high-resolution transmission electron microscopy (HR-TEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR). The gold nanoparticles had characteristic surface plasmon resonance bands at 535 nm. HR-TEM and AFM images revealed major spherical-shaped nanoparticles. The average diameter was measured to be 9.77±3.09 nm using HR-TEM images. The crystalline structure of the gold nanoparticles was confirmed through lattice fringes and circular spots within the selected area electron diffraction in the HR-TEM images along with the XRD peaks. The gold nanoparticles exhibited enhanced anticoagulant activity, as assessed by activated partial thromboplastin time. The current method is a straightforward, environmentally friendly, and inexpensive method for the production of gold nanoparticles using extracts from traditional medicinal plants.