Root canals disinfection with a paste based on metronidazole, chlorhexidine and levomycetin

Abstract

AIM. To identify the ability of an ex tempore paste with metronidazole, chlorhexidine and chloramphenicol, to disinfect root canals in patients diagnosed with “Chronic apical periodontitis” K04.5.MATERIALS AND METHODS. The study involved 30 patients, 61 root canals diagnosed with “Chronic apical periodontitis” K04.5. Treatment was carried out in accordance with clinical recommendations (treatment protocols) for the diagnosis: “Diseases of periapical tissues”, approved by Resolution No. 18 of the Council of the Association of Public Associations “Dental Association of Russia” dated September 30, 2014, updated on August 2, 2018. Biological material from the contents of the root canal was taken before and after the introduction of a paste with metronidazole, chlorhexidine and chloramphenicol into the root canal for 5 days. The following methods were used: clinical, microbiological. The total microbial number, gram-positive and gram-negative microorganisms, fungi were determined. A polymerase chain reaction (PCR) was carried out. Statistical analysis of the data included a description of accounting characteristics and assessment of the statistical significance of changes in the studied indicators. p < 0.05 was selected as the critical level of statistical significance of differences (p).RESULTS. Statistical analysis showed a pronounced, statistically significant negative dynamics for all microbiological indicators in the structure of the root canal biofilm.CONCLUSIONS. The data obtained allow us to conclude that an ex tempore paste with metronidazole, chlorhexidine and chloramphenicol can be used in the treatment of patients diagnosed with “Chronic apical periodontitis” K04.5 as an intracanal drug. It reduces all sown cultures of microorganisms in a short time (5 days).