Root Bark of Paeonia suffruticosa Extract and Its Component Methyl Gallate Possess Peroxynitrite Scavenging Activity and Anti-inflammatory Properties through NF-κB Inhibition in LPS-treated Mice.

Ji-Cheon Jeong,Hee Jung,Takako Yokozawa,Chan Park,Dong Park

doi:10.3390/molecules24193483

Abstract

A peroxynitrite (ONOO−)-generating system induced by 3-morpholinosydnonimine, was used to evaluate the ONOO− scavenging properties of plants that have been widely used as traditional medicine in Korea for the treatment of several diseases. The most effective medicinal plants were Paeonia suffruticosa Andrew, followed in order by Lonicera japonica Thunb., Curcuma zedoaria (Christm.) Roscoe, and Pueraria thunbergiana Benth. In addition, root bark of P. suffruticosa was partitioned with organic solvents of different polarities, and the ethyl acetate (EtOAc) fraction showed the strongest ONOO− scavenging activity. Methyl gallate, a plant-derived phenolic compound identified from the EtOAc fraction, exerted strong ONOO− scavenging activity. The in vivo therapeutic potential of methyl gallate was investigated using lipopolysaccharide-treated mice. Oral administration of methyl gallate protected against acute renal injury and exhibited potential anti-inflammatory properties through an increase in antioxidant activity and decrease in nuclear factor-kappa B activity.

Highlights

It is widely recognized that oxidative stress caused by excessive reactive species (RS) including reactive oxygen species (ROS) and reactive nitrogen species (RNS) is associated with the pathogenesis of diseases [1]
Antioxidants that protect against oxidative damage induced by free radicals, prevent the onset and progression of disease [4,5]
We investigated the effects of methyl gallate, a plant-derived phenolic compound found in root bark of Paeonia suffruticosa Andrew, on oxidative stress and inflammation in lipopolysaccharide (LPS)-treated mice

Summary

Introduction

It is widely recognized that oxidative stress caused by excessive reactive species (RS) including reactive oxygen species (ROS) and reactive nitrogen species (RNS) is associated with the pathogenesis of diseases [1]. Peroxynitrite (ONOO− ), a powerful oxidant formed through the reaction of nitric oxide (NO) and superoxide (O2 − ), is thought to be cytotoxic itself and is capable of breaking down into other toxic species, such as the hydroxyl radical (·OH) [2]. RNS are known to be active nuclear factor-kappa B (NF-κB), redox sensitive, and pro-inflammatory transcription factor [3]. Antioxidants that protect against oxidative damage induced by free radicals, prevent the onset and progression of disease [4,5]. Research interests are focused on the development of safe, effective, and non-toxic antioxidants.

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Results

Conclusion