Roles of modified biochar in the performance, sludge characteristics, and microbial community features of anaerobic reactor for treatment food waste

Abstract

Anaerobic digestion (AD) is a green technology widely applied to food waste treatment. Although the AD has high efficiency, instability often occurs. The main purpose of the study is to understand the mechanism of modified biochar improving AD performance. The effects of different modified biochar on the efficiency and microecology of an anaerobic reactor treating food waste were investigated. Bagasse biochar was used as the substrate to explore the effects of iron-modified (A), chitosan-modified (B), iron-chitosan-modified (C) and iron‑magnesium-chitosan-modified (D) biochar on the anaerobic digestion process, sludge characteristics and microbial community. The results show that the average COD removal efficiency of the four reactors during the last five days of the experimentation period was 86.95%, 85.90%, 92.22% and 93.29%, respectively. Adding iron‑magnesium-chitosan-modified biochar could improve the efficiency of COD removal in the anaerobic reactor under ammonia nitrogen stress. On day 10 of operation, the content of coenzyme F420 in the sludge of anaerobic reactors C and D reached to 0.44 and 0.57 mmol/g, respectively, indicating that the metal-chitosan complex biochar could promote the production of coenzyme F420 in the early stage of the experiment. Within the four anaerobic reactors, Firmicutes, Bacteroidetes, Proteobacteria and Chloroflexi were the dominant bacteria, and the abundance of Chloroflexi reached a maximum of 26.24% in the reactor C. As for archaea, Methanobacterium and Methanothrix were the most dominant accounting for 44.03%, 49.88%, 31.29%, 52.01% and 38.34%, 34.52%, 50.9%, 35.72% respectively in the four reactors. KEGG functional analysis showed that the energy metabolism of bacteria and archaea in the reactor D was the largest among the four reactors. Meanwhile, the gene abundance associated with carbohydrate metabolism and membrane transport of microorganisms in the reactor D was greater than that of other groups.