Roles of intrinsic and extrinsic tongue muscles in feeding: Electromyographic study in pigs

Abstract

The performance of tongue muscles in various feeding behaviours is not well defined. This study was undertaken to examine the role of the intrinsic and extrinsic tongue muscles during natural drinking, food ingestion and chewing. Ten 12-week-old Yucatan miniature pigs (5 in each gender) were used. Under anesthesia, fine-wire electrodes were inserted into three intrinsic (verticalis and transversus [V/T]; superior and inferior longitudinalis [SL and IL]) and two extrinsic (genioglossus [GG] and styloglossus [SG]) tongue muscles and two jaw muscles (masseter [MA] and anterior digastricus [DI]). Electromyogram (EMG) and jaw movement were recorded and synchronized when pigs were drinking water, ingesting and chewing food freely. Chewing frequency (CF), onset of activation, burst duration and integrated activity (IEMG) were assessed quantitatively, and EMG activities during drinking and ingestion were examined qualitatively. Results indicate that during chewing, the V/T and GG had one phase of activity starting at early jaw opening, and the V/T activity lasted through late of jaw closing. The SL, IL and SG had double phases with the first starting at jaw opening and the second at late jaw closing phases. The three intrinsic tongue muscles and the SG were active during 35–48% of the chewing cycle. IEMG values of the SL, IL and SG of both sides were significantly greater compared to the other muscles ( p < 0.05–0.01). Both the SL and the IL showed significantly higher activities in the contralateral than ipsilateral sides ( p < 0.05). The timing sequences of both extrinsic and intrinsic muscles were similar between ingestion and chewing, but amplitudes of the GG and IL were greatly enhanced and those of the MA and SL were reduced during ingestion. The simultaneous activation of the MA, GG and V/T were seen during drinking, along with major activity in the GG and V/T. These results suggested that the majority of activity in the intrinsic and extrinsic tongue muscles occurred during jaw opening and the occlusal phases of chewing. The activity of the GG and IL played a major role during ingestion, whereas simultaneous activation of jaw, extrinsic and intrinsic tongue muscles and major activity in the GG and V/T occurred during drinking.