Abstract
In vascular plants, cryptochromes acting as blue-light photoreceptors have various functions to adapt plants to the fluctuating light conditions on land, while the roles of cryptochromes in bryophytes have been rarely reported. In this study, we investigated functions of a single-copy ortholog of cryptochrome (MpCRY) in the liverwort Marchantia polymorpha. Knock-out of MpCRY showed that a large number of the mutant plants exhibited asymmetric growth of thalli under blue light. Transcriptome analyses indicated that MpCRY is mainly involved in photosynthesis and sugar metabolism. Further physiological analysis showed that Mpcry mutant exhibited a reduction in CO2 uptake and sucrose metabolism. In addition, exogenous application of sucrose or glucose partially restored the symmetrical growth of the Mpcry mutant thalli. Together, these results suggest that MpCRY is involved in the symmetrical growth of thallus and the regulation of carbon fixation and sucrose metabolism in M. polymorpha.
Highlights
TF Mp7g09490 Mp8g03820 Mp2g23600 Mp6g03920 Mp6g18650 Mp1g13640 Mp2g07170 Mp5g12480 Mp4g14530 Mp5g21080
Cryptochromes are phylogenetically classified into three distinctive groups, which are represented by plant cryptochromes, animal cryptochromes, and CRY-DASH, respectively [42]
Previous studies have indicated that CRY-DASH primarily plays a role in repairing cyclobutane pyrimidine dimmers, and plant cryptochromes are the major pathway for plant growth and development under blue light [42,43]
Summary
The perception and response of organisms to blue light have been a focus of scientific interest [1], especially on cryptochrome (cry), one of the blue light receptors found in the evolutionary lineages of archaea, bacteria, plants and animals [1,2,3]. PpSBP4) to induce side branch formation under blue light [15,16] These studies indicate that cryptochromes are an important factor in the transduction of blue light signal in. The liverwort Marchantia polymorpha has recently been established as a model plant species. It has a life cycle whose dominant form is haploid, and its genome shows a low Academic Editor: Stanislaw Karpinski. We found that MpCRY is involved in the regulation of photosynthesis and sucrose metabolism Together, these results provide new insights into the function of MpCRY during growth and development of M. polymorpha
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