Role of Trapped Air in the Attachment of Staphylococcus aureus on Superhydrophobic Silicone Elastomer Surfaces Textured by a Femtosecond Laser.

Abstract

Surface texturing is an easy way to control wettability as well as bacterial adhesion. Air trapped in the surface texture of an immersed sample was often proposed as the origin of the low adhesion of bacteria to surfaces showing superhydrophobic properties. In this work, we identified two sets of femtosecond laser processing parameters that led to extreme superhydrophobic textures on a silicone elastomer but showed opposite behavior against Staphylococcus aureus (S. aureus, ATCC 25923) over a short incubation times (6 h). The main difference from most of the previous studies was that the air trapping was not evaluated from the extrapolation of the results of the classical sessile drop technique but from the drop rebound and Wilhelmy plate method. Additionally, all wetting tests were performed with bacteria culture medium and at 37 °C in the case of the Wilhelmy plate method. Following this approach, we were able to study the formation of the liquid/silicone interface and the associated air trapping for immersed samples that is, by far, most representative of the cell culture conditions than those associated with the sessile drop technique. Finally, the conversion of these superhydrophobic coatings into superhydrophilic ones revealed that air trapping is not a necessary condition to avoid Staphylococcus aureus retention on one of these two textured surfaces at short incubation times.