ROLE OF THYROID HORMONES IN GONADAL SEX DIFFERENTIATION OF ZEBRAFISH

Abstract

Event Abstract Back to Event ROLE OF THYROID HORMONES IN GONADAL SEX DIFFERENTIATION OF ZEBRAFISH Prakash Sharma1* and Reynaldo Patiño2 1 Texas Tech University, Department of Biological Sciences, United States 2 U.S. Geological Survey, Texas Cooperative Fish & Wildlife Research Unit, Texas Tech University, United States An earlier study from our laboratory showed that treatment of larval zebrafish with perchlorate, a thyroid hormone (TH) synthesis inhibitor, biased the sex ratio toward females and that treatment with perchlorate + TH biased the ratio towards males. The objective of this study is to determine if the influence of early manipulation of TH condition on gonadal sex persists through the early adult stage. Three-day postfertilization (dpf) larvae were reared for 30 days in control (reconstituted water), 0.82 mM perchlorate, 0.15 and 0.3 mM methimazole, and 1 and 10 nM TH (T4) solutions. Sex ratio and thyroid follicle cell height were histologically determined at 45 and 60 dpf. Sex ratios were biased toward males in the TH treatments and toward females in the perchlorate treatment at both sampling times. Methimazole treatments biased sex ratios toward females only at 45 dpf. Perchlorate treatment increased thyroid follicular cell height at both 45 and 60 dpf, but methimazole treatment increased thyroid follicular cell height only at 45 dpf. Results of this study confirmed that thyroid endocrine status dictates the direction of gonadal sex differentiation in zebrafish. However, the persistence of the effect of TH synthesis inhibitors on thyroid condition and sex ratio differed between the inhibitors; namely the effects of methimazole on thyroid condition and gonadal sex seemed to be relatively quickly reversible. This differential response may provide a useful tool to further study and understand TH-dependent mechanisms of gonadal sex differentiation in zebrafish. (Supported by intramural funding from Texas Tech University and Texas Cooperative Fish and Wildlife Research Unit). Keywords: Epithelial Cell Height, Sex ratio, Thyroid Hormones Conference: NASCE 2011: The inaugural meeting of the North American Society for Comparative Endocrinology, Ann Arbor, United States, 13 Jul - 16 Jul, 2011. Presentation Type: Poster Topic: Thyroid Citation: Sharma P and Patiño R (2011). ROLE OF THYROID HORMONES IN GONADAL SEX DIFFERENTIATION OF ZEBRAFISH. Front. Endocrinol. Conference Abstract: NASCE 2011: The inaugural meeting of the North American Society for Comparative Endocrinology. doi: 10.3389/conf.fendo.2011.04.00066 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 22 Jul 2011; Published Online: 09 Aug 2011. * Correspondence: Mr. Prakash Sharma, Texas Tech University, Department of Biological Sciences, Lubbock, Texas, 79409, United States, prakash.sharma@ttu.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Prakash Sharma Reynaldo Patiño Google Prakash Sharma Reynaldo Patiño Google Scholar Prakash Sharma Reynaldo Patiño PubMed Prakash Sharma Reynaldo Patiño Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.