Role of the High‐Conductance Calcium‐Activated Potassium Chanel in first order mesenteric artery of hypercaloric diet rat model

Abstract

Obesity has been identified like as a risk factor of metabolic syndrome. The High‐Conductance Calcium‐Activated Potassium Channel (BKCa) in vascular smooth muscle cells regulates membrane potential and control myogenic tone.Objectiveevaluated the role of the BKCa channel in first order mesenteric arteries of rats feed with a hypercaloric diet for 18 and 28 weeks.MethodologyWe performed isometric tension experiments in first and second order mesenteric arteries rings and Patch Clamp recordings of vascular smooth muscle cells isolated of the same vascular bed.ResultsThe rats feed with a hypercaloric diet for 18 weeks shows an augmented un percentage of adipose tissue, triglycerides, glucose tolerance curve, blood pressure and more relaxation to BKCa channel activator NS11021 un rings precontracted with phenylephrine. At 28 weeks of evolution, an increase in percentage of adipose tissue with respect to body weight was observed, as well as an increase in blood pressure, Regarding BKCa channel function, an increase in current density was observed in the experimental group with respect to the control group.ConclusionChanges have been developed at the metabolic level in the groups of the hypercaloric diet, with changes in the BKCa channels, a gain of function in first order mesenteric rings of the 18 weeks group and in cells isolated from these arteries at 28 weeks group. Nevertheless, at 28 weeks of evolution, first order mesenteric artery rings no longer showed the gain in function observed in the previous group, which suggest a compensatory mechanism maybe associated to aging.