Abstract

Sarcoplasmic reticulum (SR)‐plasma membrane (PM) contact sites in vascular smooth muscle (VSM) cells bring the SR Ca2+ release channels into proximity of plasma membrane large‐conductance Ca2+‐activated K+ (BKca) channels and regulate intracellular Ca2+ through BKca‐mediated membrane hyperpolarization. Loss of BKca channel activity leads to increased intracellular Ca2+ concentration, vascular hypercontractility, and activation of hypertrophic genes. We hypothesized that the loss of close contact between the SR and the PM contributes to the development of vascular hypercontractility and hypertrophy in the Spontaneously Hypertensive Rat (SHR). To evaluate this hypothesis, we compared BKca channel activity in response to the activation of inositol 1,4,5‐trisphosphate receptor (IP3R), an SR Ca2+ channel between SHR and normotensive Sprague Dawley (SD) rats. We found that in endothelium‐denuded pressurized mesenteric arteries, paxilline (1μM), a selective BKca channel blocker, significantly increased norepinephrine (NE, 0.5μM)‐induced vasoconstriction in SD arteries (n=5) by ~15% (% initial diameter from 78.58±3.93 to 66.77±3.07). However, paxilline failed to increase NE‐induced vasoconstriction in SHR arteries (Figure 1). Paxilline treatment significantly augmented the hypertrophic effect of angiotensin II (ANG II) in SD, where the total cell area of paxilline treated ANG II group was ~11% (from 3471.48±121.35 to 3854.86±129.99μm2) greater compared to the non‐paxilline‐treated ANG II group. In contrast, the paxilline‐induced increase in hypertrophic response was absent in SHR. Similarly, in WST‐1 cell proliferation assay, paxilline treatment significantly increased ANG II‐induced proliferation in SD by ~15% (Absorbance(A440nm‐A630nm) from 0.15±0.01 to 0.17±0.01) while having no effect on cell proliferation in SHR. Next, we compared the effect of 5‐HT on BKca channel activity between SHR and SD rat mesenteric VSM cells using patch‐clamp whole‐cell mode. 5‐HT activates IP3Rs by stimulating the production of IP3 in VSM cells. At +60mV, 5‐HT (0.3μM) significantly increased BKca current density at +60mV in SD VSM cells from 50.78±2.19 to 69.40±5.50 pA/pF (n=6 cells, P<0.05) but the increase in current density was non‐significant in SHR (from 36.77±3.11 to 47.45±8.83 pA/pF, n=6 cells, Figure 2). We also examined the Ca2+‐ and voltage‐sensitivity of BKca channels using inside‐out patch‐clamp. BKca channels in VSM cells isolated from mesenteric arteries of SHR and SD rats displayed identical Ca2+‐ and voltage‐sensitivity. In conclusion, our data suggest a lack of BKca activation in response to IP3R activation in VSM cells ofSHR, which may be due to the loss of close contact between the SR and the PM.

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call