Abstract
The aim was evaluate if the alterations in ECVE observed during normal and hypertensive pregnancy is parallel with changes in the mRNA expression levels of renal transporters. Wistar rats divided in control group(C,n=5), pregnancy(P,n=5), L-NAME (L,n=6)(50mg/Kg/day) and Pregnancy+L-NAME(PL,n=6). Mean arterial pressure(MAP), heart rate(HR), stroke volume(SV), total peripheral resistence(TPR) and cardiac output(CO) were mensured at 14th pregnancy day. mRNA expression of Na/H(NHE3), Na/K/2Cl(BSC), K channel(ROMK2) and the aquaporin2(AQP2) tranporters were analyzed in the kidney tissue by RT-PCR. P group showed CO higher then C rats(P 107±7; C 84±5 ml/mim,p<0,05) without elevation in blood pressure. L group showed CO lower then C group(L 44,1±2,2 vs C 84,5±1,2; p<0,05)with significant elevation in MAP. LP group showed higher in TPR when compered with P group(LP 3,5±0,4 vs P 1,2±0,1; p<0,05)and decrease in SV. P rats showed decrease in ROMK2 mRNA expression(P 0,3 vs C 1,8; p<0,05)and increase in AQP2(P 3,5 vs C 1,3; p<0,05). L group showed significant increase in AQP2 expression(2,6) when compared with C(1,3) and LP(1,2) groups (p<0.05). LP showed significant decrease in BSC and NHE3 expression compared with P group. The hypertension by NO synthesis inhibition blunted systemic hemodinamic adaptations during the pregnancy. The pregnant hypertensive rats presented nephron transporters alterations suggesting a variation on tubular transporter could mediate the hemodynamic adaptations, also potencialy mediated by NO during pregnancy. Financial Support: FAPESP, CNPq, CAPES
Published Version
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