Abstract
Nanopore-based detection of macromolecules has gained interest in recent years, because of the enormous potential of single-molecule analysis at ultra-low analyte concentrations. In this work, we describe how nanopores can be used to trap, manipulate, and study individual bimolecular complexes with control over the applied force and other experimental parameters, such as temperature and pressure. Key to our results is the combination of ultrathin nanopores and high-bandwidth electronics, which together enable high-resolution measurements. In addition, characterizing the pore's surface charge enables more quantitative assessment of the role of electroosmosis on molecular capture and transport. We will discuss specific examples of molecular transport on well-characterized nanopores in different materials. Our results represent a key advance towards more quantitative macromolecular characterization at the single-molecule level.
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