Abstract
The present study was undertaken to compare the role of direct microscopy with microscopy after cytocentrifugation during diagnosis of fungal infections. Also, the role and usefulness of different media like Sabouraud Dextrose Agar (SDA), Emmons Sabouraud Dextrose Agar (ESDA) and Inhibitory Mold Agar (IMA) for the growth of different fungi were compared to determine the most effective primary medium to be used in routine. Out of the total number of observations, 11.25% showed 2 folds increase, 28.75% showed 3 folds increase, 20% showed 4 folds increase and 40% showed 5 folds or more increase in cell density after cytocentrifugation. Also there were 3.75% of negative observations during direct microscopy while positive results were obtained on microscopy of same samples after cytocentrifugation. On studying the effect of various primary media on isolation and growth of different fungi, a total of 27 fungal isolates were recovered on culture media and identified. These isolates represented six important genera of fungi namely Candida, Penicillium, Trichophyton, Aspergillus, Cryptocococcus and Rhizopus. Out of a total of 27 fungal isolates, 66.66% fungal isolates were isolated on all the three media, 11.11% on SDA alone, 7.40% on Emmons SDA alone and 14.81% on IMA alone.
Highlights
Medical mycology has become an extremely challenging field of due to the infections caused by a wide and taxonomically diverse array of opportunistic fungi
This study was undertaken to compare direct microscopy with microscopy after cytocentrifugation and to compare the role and usefulness of different media like Sabouraud dextrose agar (SDA), Emmons SDA and Inhibitory mold agar (IMA) for the growth of different fungi and determine the most effective primary medium to be used in routine
SDA continues to have an important role in laboratory as some isolates were recovered only on SDA
Summary
Medical mycology has become an extremely challenging field of due to the infections caused by a wide and taxonomically diverse array of opportunistic fungi. Isolation and identification of the infecting organisms is extremely important for the proper diagnosis and management of infections due to not so common opportunistic fungi [1, 2]. The Cytospin system uses both the principles of centrifugation and fluid absorption to allow the deposition of a thin layer of cells on glass slide in the form of round or rectangular areas. Earlier it was used mainly for cerebrospinal fluid, but cytocentrifugation is thought to be useful for several body fluids including urine, sputum, ascites and cerebrospinal fluid [3, 4]
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