Role of PPAR Gamma in obesity and obesity induced DM type-2

Abstract

PPAR-γ plays a critical role in glucose homeostasis and is a molecular target of insulin sensitizing drugs called thiazolidinediones which are PPAR-γ ligands (3). PPAR-γ ligands also upregulate expression of adipocyte glycerol kinase, resulting into improved insulin action in target tissue such as liver and muscle (4). PPAR-γ ligand also shown to augment glucose disposal in peripheral tissues by increasing expressions of the glucose transporter genes, GLUT-1 and GLUT-4 (5 Majority of the Diabetes Mellitus Type 2 (DM type 2) patients (80%) are obese, but all obese persons do not develop DM type 2. Serum adiponectin level should be determined by level of PPAR-γ activity in adipocytes. Therefore it will be of interest to explore the difference in the level of serum PPAR-γ level between obese subjects with DM-2 and without DM-2, as both the insulin sensitizing factor’s adiponectin, and vistafin are synthesized in response to FFA. PPAR-γ complex receptor activity. Estimation of serum PPAR- level alongwith FBG was done in 90 cases, of which 30 were normal healthy subjects, 30 obese without diabetes type 2 and 30 obese with diabetes type 2 . The qualitative estimation of PPAR-γ in human serum was done by ELISA method using ELISA kit from E lab science Biotechnology co.ltd. In our study we found a significant change in the level of PPAR- between the obese with DM-2 and control subject and also between obese without DM-2 and control subject but we did not find any significant difference between obese with DM-2 and obese without DM-2 In our present study we found the Increased Levels of PPAR- in the Obese without Diabetes Mellitus and Obese with Diabetes Mellitus. The levels were 8.03ng/ml ±11.16 and 8.04ng/ml±10.94 respectively. Finally, we observed that the level of PPAR- is increased with both the groups,i.e .obese without DM-2 and obese with DM-2 in comparison to normal subjects.