Role of oxylipin on Luffa seedlings exposed to NaCl and UV-B stresses: An insight into mechanism

Abstract

Nowadays, among several abiotic stresses, salt, especially NaCl and UV-B are of major concern. They lead to deleterious effects on plant growth and ultimately affect crop productivity. The present study was planned to find out some ameliorative solution against these stresses. Here, the modulatory action of two oxylipins, namely, methyl jasmonate (MeJA) and 12-Oxo-phytodienoic acid (OPDA) on growth, photosynthetic performance, nitrate/ammonia assimilating enzymes, and nutritive values of Luffa Mill. seedlings grown under NaCl (20 and 40 mM) and/or enhanced UV-B stresses (ambient: 8.2 kJ m−2 d−1 + additional: 2.2 kJ m−2 s−1) were analyzed. Both the stresses when given alone, negatively affected the fresh mass, root/shoot ratio, leaf area, photosynthetic pigments content, photosynthetic oxygen yield and, chlorophyll a fluorescence kinetic parameter. This decline was further aggravated upon combined exposure to the stressors. However, supplementation of MeJA/OPDA effectively counteracted the negative impact on important growth-regulating processes. The activities of nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), and glutamate synthase (GOGAT) enzymes, as well as the contents of inorganic nitrogen, protein, and carbohydrate, were increased with the supplementation of MeJA/OPDA. The increase in the Na+ and Cl‾ contents due to NaCl or/and UV-B was depreciated by MeJA or OPDA. Ameliorating behaviour of MeJA or OPDA is correlated with improved photosynthetic activity and nitrogen metabolism. These findings, point out that supplementation of MeJA/OPDA, particularly OPDA more favourably regulated the growth-promoting activities, which can be linked with the mitigation of NaCl and UV-B stress.