Role of Osteogenesis of MC3T3-E1 Induced by Demineralized Tooth Block with Composite Acids: An In Vitro Study

Abstract

More and more research had focused on the osteogenesis of demineralized dentin in clinic, especially when the first application of deminerized dentin in 2008. The study tried to compare the osteogenetic ability of the demineralized dentin block, which were processed two different regents by VacuaSonic system. The extracted human permanent teeth were demineralized by two different methods. Then the MC3T3-E1 cells were invited to culture on the surface of these demineralized dentin blocks (DDB). The cell attachment, proliferation and differentiation were tested. Adhesion of MC3T3-E1 on DDM was observed using scanning electron microscopy and confocal test, the Alizarin Red S, ALP activity, and the protein of BMP-2/-7 and OCN were employed to confirm the level of cell differentiation. The P value was set at 0.05. The microfilaments established a good contact and formed a network in Group A. The Group A had more full cytoskeleton and actin stretched more obviously than Group C, the number of cells on three scaffolds were difference (p < 0 05). The MTT results showed no cytotoxicity in all experiment groups, and Group C had a significant difference in cell proliferation than other groups (p < 0 05) except for day 1. While when related to the cell differentiation, Group A showed a similar result with Group C, but in Alizarin Red S, Group A had a superior result (p < 0 05). The tooth dentin scaffold processed with composite acids in Group A presents the superiority in osteoconduction and preferable osteogenesis ability, which could be an alternative method to process the tooth scaffold.