Role of NF-κB p65 and hsp70 in colorectal adenocarcinomA

Abstract

Free radicals and inflammation in tumor microenvironment are important aspects of tumor biological activity. They activated NF-κB p65 transcription factor pathway. HSP70 is chaperone protein that regulated host of imune response to cancer cells. NF-κB p65 and HSP70 expression is associated with poor prognosis and new targeted therapy in colorectal adenocarcinoma. This study aims to analyze correlation between NF-κB p65 expression in macrophages and HSP70 in tumor cells with various T stages of colorectal adenocarcinoma. This cross-sectional study was performed on 48 paraffin blocks from colorectal adenocarcinoma at the Anatomical Pathology Laboratory of Dr. Soetomo General Academic Hospital Surabaya from January 2015 to December 2019. The samples were divided into four groups based on T stages of colorectal adenocarcinoma. Immunohistochemical staining was performed to detect NF-κB p65 expression in nucleus or cytoplasm of macrophages and HSP70 in cytoplasm of tumor cells. There was no significant difference of NF-κB p65 expression percentage in macrophages at four groups (p=0.31). There was significant differences of HSP70 expression percentage in tumor cells at four groups (p=0.014). There was no correlation between NF-κB p65 expression in macrophages and HSP70 expression in tumor cells with various T stages of colorectal adenocarcinoma (rs=0.05, p=0.97).