Protective effect of diallyl trisulfide on acute lung injury in rats

Abstract

Objective Observing the protective effect of diallyl trisulfide (DATS) on acute lung injury induced by cecal ligation and puncture (CLP). Methods The animal model of acute lung injury was induced by CLP. The 30 male SD rats were fasted 16 h before surgery, then was anesthetized by intraperitoneal injection of 10% chloral hydrate (4 ml/kg). Then all rats were randomly divided into 3 groups (n=10), sham group (group Ⅰ), CLP+normal saline (NS) group (groupⅡ) and CLP+DATS group (group Ⅲ). Rats in groupⅠ and group Ⅱ were injected with saline 10 ml/kg through caudal vein, but group Ⅲ were injected with DATS 10 mg/kg. The arterial cannulation was used in carotid artery of the rat to measure the blood gas after the surgery at 0 h (T0), 6 h (T6) and 12 h (T12). All male SD rats were subcutaneously injected with normal saline 0.05 ml/g. Subsequently, the male SD rats were sacrificed at 12 h after operation by the blooding and collected samples of lung tissue. The lung tissue wet/dry weight ratio (W/D), myeloperoxidase (MPO) activity, malondialdehyde (MDA), superoxide dismutase (SOD) and nitric oxide (NO) levels, expression of nuclear factor-κB(NF-κB) P65 and IκB-α in the cytoplasm of lung tissue, expression of NF-κB P65 in the nucleus of lung tissue were measured. Pathological changes of lung tissue was observed under light microscope. Another 30 male SD rats were grouped randomly and received the same experiment methods. The survival rate at 24 h was observed. Results Compared with group Ⅰ, the arterial partial pressure of oxygen (PaO2) and activity of SOD were significantly decreased (P<0.05), but the arterial partial pressure of carbon dioxide (PaCO2), lung tissue W/D, activity of MPO, concentration of MDA and NO in the group Ⅱ and group Ⅲ at T6 and T12 were significantly increased (P<0.05). Inflammatory reaction was significantly increased under light microscope in the group Ⅱ. The expression of cytoplasmic NF-κB P65 and IκB-α was decreased (P<0.05), but the expression of nucleus NF-κB P65 was increased in the group Ⅱ(P<0.05). The survival rate of the group Ⅱ in 24 h was lower than the rate in group Ⅰ (P<0.05). Compared with group Ⅱ, the arterial PaO2 and activity of SOD was increased (P<0.05), but the arterial PaCO2, lung tissue W/D, activity of MPO, concentration of MDA and NO were decreased in the group Ⅲ at T6 and T12(P<0.05). Inflammatory reaction was significantly decreased under light microscope in group Ⅲ. The expression of cytoplasmic NF-κB P65 and IκB-α were increased (P<0.05), but the expression of nucleus NF-κB P65 were decreased in group Ⅲ (P<0.05). The survival rate of the group Ⅲ in 24 h was improved (P<0.05). Conclusions DATS has a protective effect on acute lung injury in SD rats. Key words: Diallyl trisulfide; Cecum; Perforation of intestine; Acute lung injury