Abstract
Objective To study the role of serine protease encoding gene prtP carried by Lactobacillus paracasei (Lp) in regulating mouse intestinal immunity. Methods Female BALB/c mice were randomly divided into seven groups and respectively administered with PBS, medium (5×108 CFU/ml) and high dose (1×109 CFU/ml) of Lp, Lp△prtP and ReLp△prtP for 21 consecutive days by intragastric gavage. Parameters including immune organ index, splenic lymphocyte transformation rate, energy metabolism in celiac macrophages, phagocytic ability of celiac macrophages and cytokines were detected. Quantitative changes in CD11c+ CD80+ cells in culture supernatants of splenic mononuclear cells were analyzed by flow cytometry. Results Compared with Lp△prtP, Lp could significantly promote the natural transformation of splenic lymphocytes, increase the level of energy metabolism in celiac macrophages, enhance the phagocytic ability of celiac macrophages, and up-regulate the expression of IFN-α, IL-10 and IFN-γ and the percentage of CD11c+ CD80+ cells. Conclusion Serine protease encoding gene prtP in Lp can regulate the mucosal immune system in mice. Key words: Lactobacillus paracasei; prtP gene; Intestinal immunity
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