Role of iron(II) sulfide in autotrophic denitrification under tetracycline stress: Substrate and detoxification effect

Abstract

Autotrophic denitrification using inorganic compounds as electron donors has gained increasing attention in the field of wastewater treatment due to its numerous advantages, such as no need for exogenous organic carbon, low energy input, and low sludge production. Tetracycline (TC), a refractory contaminant, is often found coexisting with nutrients (NO3− and PO43−) in wastewater, which can negatively affect the biological nutrient removal process because of its biological toxicity. However, the performance of autotrophic denitrification under TC stress has rarely been reported. In this study, the effects of TC on autotrophic denitrification with thiosulfate (Na2S2O3) and iron (II) sulfide (FeS) as the electron donors were investigated. With Na2S2O3 as the electron donor, TC slowed down the nitrate removal rate, which decreased from 1.32 to 0.18 d−1, when TC concentration increased from 0 mg/L to 50 mg/L. When TC concentration was higher than 2 mg/L, nitrite reduction was seriously inhibited, leading to nitrite accumulation. With FeS as the electron donor, nitrate removal was much more efficient under TC-stressed conditions, and no distinct nitrite accumulation was observed when the initial TC concentration was as high as 10 mg/L, indicating the effective detoxification of FeS. The detoxification effects in the FeS autotrophic denitrification system mainly resulted from the rapid adsorption of TC by FeS and effective degradation of TC, as proven by a relatively higher living biomass area. This study offers new insights into the response of sulfur-based autotrophic denitrifiers to TC stress and demonstrates that the FeS-based autotrophic denitrification process is a promising technology for the treatment of wastewater containing emerging contaminants and nutrients.