Abstract
The retention of various saturated and unsaturated solutes in reversed-phase liquid chromatography (RPLC) was investigated for various saturated and unsaturated packings in mobile packings in mobile phase mixtures of various compositions of methanol and acetonitrile. Non-aqueous eluents containing acetonitrile have a lower solvent strength than eluents containing methanol for saturated alkanes, alcohols and triglycerides, as expected from the solvophobic theory of retention. Very peculiar retention behaviours are observed when unsaturated solutes are analysed on aromatic stationary phases and/or when mobile phase containing acetonitrile is used with either an aromatic stationary phase or with unsaturated solutes or both. All these observations can be rationalized by taking into account the effect of π-π interactions between the π-electron systems of the solute, the stationary phase and the mobile phase. The influence of the residual silanols on silica-based RPLC supports on the retention of alcoholic solutes in pure acetonitrile is demonstrated. The influence of the concentration of a charge-transfer complexing agent (silver nitrate) in the mobile phase on the retention of unsaturated triglycerides was studied in a methanolic eluent and compared with that in a mobile phase containing acetonitrile.
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