Abstract
Chronic GvHD is important for the long-term outcome of patients after allogeneic stem cell transplantation. Chronic sclerodermatous GvHD is similar to autoimmune scleroderma in clinical and histological characteristics. In patients with autoimmune scleroderma, disease activity correlates closely with extended collagen synthesis. To elucidate the mechanisms of extended collagen synthesis in patients with csGvHD fibroblasts were cocultured with primed non-adherent mononuclear cells from healthy donors in the presence/absence of certain stimuli. Collagen synthesis was measured by specific RIA for the N-terminal propeptide of Collagen type I. Results show a significant increase in collagen type I synthesis after coculture with IL-4 primed non-adherent mononuclear cells and allogeneic stimulus. This correlates with down-regulation of IFN-γ. Substitution of IFN-γ could diminish the collagen stimulating effect in 2/3 patients.
Published Version
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