Abstract
AbstractFormaldehyde, an intermediate in methanol oxidation, plays an important role in controlling methanol utilization by microorganisms which fix carbon via the ribulose monophosphate (RMP) cycle. The extracellular formaldehyde concentration profiles in batch cultures at low and high starting methanol concentrations with and without the presence of semicarbazide in the media, suggest the role played by formaldehyde. The impact of formaldehyde on growth is demonstrated by the time dependent and the initial methanol concentration‐dependent cell‐mass‐yield coefficient. Kinetic studies of the enzymes involved in the oxidation and incorporation of C1 units suggest that enzyme inhibition cannot account for the observed growth inhibition. A mechanism is proposed to explain methanol and formaldehyde utilization that stresses the transcriptional regulation of mass flow around formaldehyde in assimilation and oxidation pathways through repression and induction.
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