Abstract
The human LAT gene encodes an intracellular adaptor protein that is crucial for the development and/or function of T cells, mast cells, Natural Killer cells, and megakaryocytes/platelets. Previously, the lab identified highly conserved binding sites for the Ets and Runx family of transcription factors within the LAT proximal promoter region. Results from the electrophoretic mobility shift assay (EMSA) and transient transfections suggested that Ets1, Elf1 and Runx1 bind to these sites to modulate LAT transcription. More recently, the lab performed siRNA mediated knockdown experiments to investigate the functional contribution of Ets1, Elf1, and Runx1 in regulating LAT gene expression in T cells and mast cells. Surprisingly, knockdown of these transcription factors did not have an appreciable effect on LAT expression. Based on these results, the lab used chromatin immunoprecipitation assays (ChIP) paired with quantitative PCR to ascertain the binding of these transcription factors as well as others to the LAT promoter region. Results from these experiments will be presented.
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