Abstract
Photosynthetic membrane fragments were prepared from Anacystis nidulans by French pressure cell disruption. Ascorbate was required to stabilize photophosphorylation activity in membranes kept at near 0°C. Divalent cations were required during mechanical disruption and during assays for Photosystem II activity, with Ca 2+ serving best. The rate of photophosphorylation was severely inhibited by Ca 2+ during assays. Results suggest that best rates are achieved when photosynthetic membranes contain Ca 2+ exposed to the interior surface, facilitating Photosystem II activity, and Mg 2+ exposed to the exterior surface during assays, facilitating photophosphorylation activity.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
