Abstract
Objective To investigate the role of the cyclin D1 in inhibition of human gallbladder carcinoma(hGBC) cells by arsenic trioxide(As_2O_3). Methods After the hGBC cells were cultured with different concentrations of As_2O_3, the proliferative activity of cells was detected by MTT methods, the cell cycle status determined by flow cytometry as well as the protein and mRNA expression of Cyclin D1,D2,D3,CDK4 and CDK6 analyzed by Western blot and RT-PCR. hGBC cells were transiently transfected with overexpression of Cyclin D1 derived from the constructed plasmid. Meanwhile, the As_2O_3-induced alteration of cell cycle was detected. Results The As_2O_3could inhibit the growth of cells in a time-and dose-dependent manner, make cells arrested in G_1,and downregulate the expression of Cyclin D1. Moreover, Cyclin D1 overexpression played the negative role in As_2O_3-induced alteration of cell cycle. Conclusion Cyelin D1 plays an important role in inhibition of hGBC cells by As_2O_3. Key words: Gallbladder neoplasm; Cyclin D1; Arsenic trioxide; Human gallbladder carcinoma cell
Published Version
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