Role of CTRP3, CTRP9 and MCP-1 for the evaluation of T2DM associated coronary artery disease in Egyptian postmenopausal females.

Sara F Ahmed,Marwa I Shabayek,Mostafa E Abdel Ghany,Hala O El-Mesallamy,Mohamed H El-Hefnawy,Jonathan M Peterson

doi:10.1371/journal.pone.0208038

Sara F Ahmed, Marwa I Shabayek + Show 4 more

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https://doi.org/10.1371/journal.pone.0208038

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Abstract

C1q complement/tumor necrosis factor (TNF)—related protein (CTRP) family comprises of 15 proteins that posses important implications in energy homeostasis, infection and inflammation. However, their roles in diabetes mellitus (DM) and its vascular complications have not been completely assessed. This works aims to study the association of two CTRPs; 3 and 9, with pro-inflammatory cytokine monocyte chemoattractant protein-1 (MCP-1), and biochemical parameters of type 2 diabetes (T2D), dyslipidemia and coronary artery disease (CAD).Methods: Biochemical markers and serum levels of CTRPs and MCP-1 were measured in 86 postmenopausal females. Subjects were divided over four groups; 13 apparent healthy subjects as control (group I), 29 patients with CAD (group II), 29 patients with T2D ≥5 years (group III) and 15 patients with CAD secondary to T2D (group IV). Serum CTRP3, CTRP9, MCP-1 and insulin were measured by ELISA.Results: Serum CTRP3 levels were found to be significantly higher in group III and IV, whereas, it was significantly lower in group II on comparing to group I. While, CTRP9 levels were significantly decreased in group II, III and IV on comparing to group I. MCP-1 levels were found to be significantly increased in groups II, III and IV on comparison with group I. Both CTRPs were significantly negatively correlated with each other. While MCP-1 was significantly correlated negatively to CTRP9.Conclusion: This study associates the possible role of CTRP3, CTRP9 and MCP-1/CCL2 in the diagnosis/prognosis of CAD complication in T2D postmenopausal females.

Highlights

Diabetes mellitus (DM) is a chronic metabolic disease, its development involves several pathogenic processes [1]
This correlation proposed that the elevated CTRP3 levels in type 2 diabetes (T2D) could be a compensatory mechanism developed in long standing DM to decrease the state of hyperglycemia through altering the increased insulin resistance (IR) and decreased insulin sensitivity
Our results showed that monocyte chemoattractant protein-1 (MCP-1) was positively associated with body mass index (BMI), FBG, HbA1c %, insulin, total cholesterol (TC), TG, low density lipoprotein cholesterol (LDL-C), TC/high density lipoprotein cholesterol (HDL-C), LDL-C /HDL-C and HOMA-IR, while negatively associated with age, T2D duration, HDL-C, QUICKI and CTRP9

Summary

Introduction

Diabetes mellitus (DM) is a chronic metabolic disease, its development involves several pathogenic processes [1]. All of these processes results in loss of β-cell mass and/or. Association of CTRP3, CTRP9 and MCP-1 with CAD and diabetes in women function that are clinically manifested as hyperglycemia [2]. The number of diabetic patients in 2015 were estimated to be around 415 million people and expected to reach 642 million by the year 2040, which represents an exponential increase mainly in developing countries[[3]. Among the several types of DM, type 2 diabetes (T2D) is a progressive pandemic that accounts for more than 90% of all cases, representing an indisputable threat to the public health[[4]. T2D is a complex polygenic disorder which is a result of the combination of hereditary components and secondary contributing factors which include; obesity, lifestyle, birth weight and stress [5]

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