Abstract
Abstract: Chromatography is a widely used analytical technique for separating and analyzing compounds present in complex mixtures. It operates on the principle of differential distribution of components between a stationary phase and a mobile phase, enabling the separation of substances based on their varying affinities for these phases. Over the years, chromatography has evolved into a versatile and indispensable tool in various fields, including chemistry, biochemistry, pharmacology, food science, environmental analysis, and clinical diagnostics. The primary types of chromatography include gas chromatography (GC), liquid chromatography (LC), ion-exchange chromatography (IEC), affinity chromatography, and size exclusion chromatography (SEC). Each type is suited to different applications, based on factors like the nature of the analytes, separation efficiency, and detection sensitivity. High-performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UHPLC) are particularly renowned for their resolution and speed, making them indispensable in pharmaceutical and clinical settings. This review provides an overview of the principles, types, applications, and recent advancements in chromatography. It highlights the integration of chromatography with other techniques, such as mass spectrometry (MS) and spectroscopy, to enhance its sensitivity and precision. Additionally, it discusses emerging trends, such as miniaturization and automation that are shaping the future of chromatographic analysis in research, quality control, and diagnostics.
Published Version
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