Abstract
Objective To investigate the role of Calcium/calmodulin-dependent serine protein kinase (CASK) in insulin granules secretion. Methods Interference or overexpression of CASK in combination with forskolin or nifidipine treatment were applied to explore the role of CASK in insulin granules secretion in pancreatic β-cell line (INS-1 cells). The anchoring and number of insulin granules were detected using electro microscope after CASK interference in primary islets. The t test was used for pairwise comparison, and differences among groups were compared by analysis of variance. Results (1) Knockdown of CASK suppressed the effect of forskolin mediated insulin secretion [(4.5 ± 0.4)% vs (2.7 ± 0.6)%, t=6.019, P 0.05]. (3) The anchoring of insulin granules to cell member was dramatically attenuated after CASK interference, while the total number of insulin granules was not statistically different between groups [(140 ± 30) vs (123 ± 45), t=0.44, P>0.05]. Conclusion CASK is involved in foskoline induced insulin secretion. CASK may play a role in the downstream of calcium channel. CASK is probably involved in the anchoring of insulin granules. Key words: Insulin; Calcium/calmodulin-dependent serine protein kinase; Insulin-secreting cells
Published Version
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