Abstract
Author(s): McCammon, Julia Grace; Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Mondal, Sambhu Charan; Jana, Snehasis | Abstract: Study the impact of Biofield Treated vitamin D3 and DMEM on bone health in human bone osteosarcoma cells (MG-63) was investigated. The test items (TI), were distributed into two parts. One part of each sample was received the Consciousness Energy Healing Treatment by Julia Grace McCammon, and those samples were labeled as Biofield Treated (BT) samples, while other parts of each sample were denoted as untreated test items (UT), where did not provide any types of treatment. Cell viability showed test samples were found as safe in tested concentrations. ALP was significantly increased by 210.4% and 221.6% in UT-DMEM+BT-TI and BT-DMEM+BT-TI respectively at 0.1 µg/mL, while increased by 207.9% in BT-DMEM+UT-TI compared to UT-DMEM+UT-TI. Collagen was significantly increased by 158.46%, 129.23%, and 138.46% in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI respectively at 1 µg/mL compared to untreated. Moreover, level of collagen was significantly enhanced by 101.37%, 157.53%, and 176.71% in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI respectively at 10 µg/mL compared to untreated. Besides, the percent of bone mineralization was remarkably increased by 70.59% and 151.18% in the BT-DMEM+UT-TI and BT-DMEM+BT-TI respectively at 100 µg/mL, while increased by 117.17% in UT-DMEM+BT-TI at 1 µg/mL than untreated. Altogether, the Biofield Energy Treated vitamin D3 was significantly improved the bone cell growth-related parameters. It could be used as an alternative supplement for vitamin D3 deficiency on various bone-related disorders (osteoporosis, low bone density, Paget’s disease, rickets, osteomalacia), stress, aging, autoimmune, and inflammatory disorders.
Highlights
In most of the living vertebrates, vitamin D3 plays a vital role maintaining a healthy skeletal system and is essential for bone cell growth and development
The level of alkaline phosphatase (ALP) was significantly increased by 210.4% and 221.6% in the untreated test items (UT)-Dulbecco’s Modified Eagle’s Medium (DMEM)+BTTest item and Biofield Treated (BT)-DMEM+BT-Test item groups, respectively at 0.1 μg/mL with respect to the untreated DMEM (UT-DMEM)+UT-Test item group
The Consciousness Energy Treated (The Trivedi Effect®) vitamin D3 showed an improved synthesis of ALP in the human osteosarcoma cells with respect to the untreated item items group, which might be advantageous to maintain a healthy skeletal structure for the patients suffering from various bone-related disorders
Summary
In most of the living vertebrates, vitamin D3 (cholecalciferol) plays a vital role maintaining a healthy skeletal system and is essential for bone cell growth and development. It is synthesized in the presence of sunlight [1]. Decrease in muscle strength with definite alteration in the immune and inflammatory responses [6,7] This metabolic bone disorders are mainly prevalent in post-menopausal women, due to rapid bone loss and change of endocrine secretions in post-menopausal women leads to an increased risk of fractures [8].
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