Abstract
ABSTRACT The purpose of this study was to evaluate microcosm methods that can be used to predict the in situ anaerobic reductive dechlorination of trichloroethene (TCE) in bedrock aquifers with and without amendments. Microcosm conditions (e.g., surface area:volume ratio, initial TCE concentration [C 0], incubation temperature) were selected to simulate in situ conditions at the site. The effect of rock media in the microcosms as a source of surface area and nutrients was also assessed. Comparison was made between microcosms constructed with sterile rock media and rock media submerged for 45 days in a groundwater well within a TCE plume for field colonization. Statistically significant biotic degradation of TCE was observed in microcosms with both groundwater only and non-field-colonized rock media, when amended with lactate. Measured half-lives were similar to half-lives observed in field conditions. Normalizing biotic results to abiotic results provides a method that deducts the abiotic losses and allows complete comparison between sample rounds, assuming samples were randomly selected for analysis within the sample round to avoid systematic errors or variation.
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