Abstract
Salinity is one of the most prominent abiotic factors, which greatly influence reproduction, development, growth, physiological and metabolic activities of fishes. Spotted sea bass (Lateolabrax maculatus), as a euryhaline marine teleost, has extraordinary ability to deal with a wide range of salinity changes. However, this species is devoid of genomic resources, and no study has been conducted at the transcriptomic level to determine genes responsible for salinity regulation, which impedes the understanding of the fundamental mechanism conferring tolerance to salinity fluctuations. Liver, as the major metabolic organ, is the key source supplying energy for iono- and osmoregulation in fish, however, little attention has been paid to its salinity-related functions but which should not be ignored. In this study, we perform RNA-Seq analysis to identify genes involved in salinity adaptation and osmoregulation in liver of spotted sea bass, generating from the fishes exposed to low and high salinity water (5 vs 30ppt). After de novo assembly, annotation and differential gene expression analysis, a total of 455 genes were differentially expressed, including 184 up-regulated and 271 down-regulated transcripts in low salinity-acclimated fish group compared with that in high salinity-acclimated group. A number of genes with a potential role in salinity adaptation for spotted sea bass were classified into five functional categories based on the gene ontology (GO) and enrichment analysis, which include genes involved in metabolites and ion transporters, energy metabolism, signal transduction, immune response and structure reorganization. The candidate genes identified in L. maculates liver provide valuable information to explore new pathways related to fish salinity and osmotic regulation. Besides, the transcriptomic sequencing data supplies significant resources for identification of novel genes and further studying biological questions in spotted sea bass.
Highlights
Salinity is one of the most important environmental factors that greatly affect the survival, reproduction, growth, development and physiological functions of teleosts
After de novo assembly analysis based on all Illumina clean reads, a total of 197,550 transcripts were generated for L. maculates with N50 size of 1,536 bp (Table 2)
RNA sequencing technology (RNA-Seq) analyses have been accepted as a robust approach to assess transcriptional responses to different experimental conditions, especially in non-model organisms for which no reference genome is available [17]
Summary
Salinity is one of the most important environmental factors that greatly affect the survival, reproduction, growth, development and physiological functions of teleosts. The tolerance to salinity variation depends on their ability of osmotic regulation [1]. RNA-Seq of salinity regulated liver transcriptome in spotted sea bass. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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