Abstract
A survey of RNA editing of miRNAs from ten human tissues indicates that RNA editing increases the diversity of miRNAs and their targets.
Highlights
MicroRNAs are short RNAs of around 22 nucleotides that regulate gene expression
To search for RNA-editing sites in human miRNAs, PCR product sequencing was performed from matched total cDNA and genomic DNA isolated from adult human brain, heart, liver, lung, ovary, placenta, skeletal muscle, small intestine, spleen and testis
Total cDNA sequence traces were compared with genomic DNA sequence traces from the same individual, and adenosine to inosine (A-to-I) editing was identified as an A in the genomic DNA sequence compared with a novel G peak at the equivalent position in the total cDNA sequence
Summary
MicroRNAs (miRNAs) are short RNAs of around 22 nucleotides that regulate gene expression. MicroRNAs (miRNAs) are short (around 20-22 nucleotides) RNAs that post-transcriptionally regulate gene expression by base-pairing with complementary sequences in the 3' untranslated regions (UTRs) of protein-coding transcripts and directing translational repression or transcript degradation [1,2,3,4,5]. MiRNAs are transcribed by RNA polymerase II into long primary miRNA (pri-miRNA) transcripts which are capped and polyadenylated [13,14]. Genomic analyses indicate that many miRNAs overlap known protein coding genes or non-coding RNAs [15], and that many are in evolutionarily conserved clusters with other miRNAs [16]. Intronic miRNAs share expression patterns with adjacent miRNAs and the host gene mRNA indicating that they are coordinately coexpressed [17]
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