Abstract
RNA binding proteins can play important roles in gene regulation and are also associated with a large number of human diseases. Identifying the sets of RNAs to which these proteins bind is critical to understanding their normal functions and roles in disease . Nova is a tissue-specific splicing factor involved in the disease paraneoplastic opsoclonus myoclonus ataxia (POMA), in which patients cannot inhibit movement and suffer uncontrollable shaking. Ule et al. developed an ultraviolet cross-linking and immunoprecipitation method to purify Nova-RNA complexes. Nova regulates a set of RNA targets that code for proteins involved in neuronal inhibition or that function at the synapse, consistent with POMA's being a disorder of motor inhibition. J. Ule, K. B. Jensen, M. Ruggiu, A. Mele, A. Ule, R. B. Darnell, CLIP identifies Nova-regulated RNA networks in the brain. Science 302 , 1212-1215 (2003). [Abstract] [Full Text]
Published Version
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