Risks’ Stratification of Metabolic Disorders and Arterial Hypertension Depending on the NOS3 (RS2070744) and GNB3 (RS5443) Genes’ Allelic State

Abstract

Early diagnosis of essential arterial hypertension (EAH), the search for risk factors for its occurrence and severity, which determine the metabolome and epigenetic structures activity, is an urgent task of modern medicine. The objective: to assess the risks of metabolic disorders and the EAH occurrence, depending on the NOS3 (rs2070744) and GNB3 (rs5443) genes’ allelic state. Materials and methods. 100 patients with EAH and 48 practically healthy (control group) were enrolled in the study. All participant underwent clinical and laboratory examinations. Metabolic changes were examined by blood glucose, total cholesterol (TC), triacylglycerol (TG), high and low density lipoprotein cholesterol (HDL-C, LDL-C), Atherogenicity index (AI). The NOS3 (rs2070744) and GNB3 (rs5443) genes were studied by real-time PCR. Risks, prognoses were determined by the clinical epidemiology method. Results. The risk of metabolic disorders (dyslipidemia and hyperglycemia) in EAH patients does not depend on NOS3 gene polymorphism (rs2070744). However, the mutational T-allele of the GNB3 gene (825C>T) presence in the patients’ genotype increases the risk of hyperlipidemia due to atherogenic LDL-C 8.5 times [OR=8.45; OR 95%CI:0.99–72.70; p=0.05], with the CC-genotype protective role [OR=0.12; OR 95%CI:0.01–1.0; p=0.048]. The overall risk of EAH in the examined population increases with fasting hyperglycemia 9 times [OR 95%CI:2.86–27.08; p<0.001], with hypertriglyceridemia (>1.70 mmol/l) – 3 times [OR 95%CI:1.23–5.56; p=0.009] and with a decrease in HDL-C (<1.2 mmol/l) – more than 3.5 times [OR 95%CI:1.46–8.71; p=0.003], respectively. Conclusion. The risk of metabolic disorders increases in EAH patients with the T-allele of the GNB3 gene (825C>T) 8.5 times with the lowest probability of such changes in the CC-genotype carriers [OR=0.12; p=0.048].