Abstract
The time-correlated single photon counting technique was applied to the measurement of photosensitized singlet oxygen luminescence induced by flash laser excitation. Rise and decay kinetics of this luminescence has been measured with 10 ns resolution in aqueous solutions of tetra ( p-sulfophenyl)porphyrin at various oxygen pressures. The rise kinetics are shown to be determined by the process of energy transfer to oxygen from the triplet state of the photosensitizer. The lifetime of singlet molecular oxygen in water was measured with high precision: τ Δ = 3.09±0.06 μs. The rise and decay kinetics of the singlet oxygen luminescence in aqueous suspensions of porphyrin-containing yeast cells were detected.
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