Abstract
The ribosomal RNA gene contents of wheat aneuploid lines, differing in the numbers of 5 A, 5B, 5D, 6A, 6B and 6D chromosomes, have been estimated by ribosomal RNA/DNA hybridisation. These and other estimates on intervarietal substitution lines, provide some evidence for ribosomal RNA genes being localised on chromosomes 5D and 6B. Chromosome 6B of the variety Chinese Spring possesses approximately 5500 rRNA genes, 60 per cent of the total ribosomal RNA gene complement of the variety. In the variety Hold-fast, chromosome 6B possess approximately 2000 ribosomal RNA genes. This value comes from examining interspecies substitution lines in which chromosomes 6A, 6B and 6D are replaced by rye chromosomes. The approximate number of ribosomal RNA genes on the four nucleolar organiser chromosomes of the variety Chinese Spring are presented.
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