Abstract
Ribonucleic acid (RNA) and protein synthesis in the mouse endometrium during activation of the implantation window was studied. Ovulations of BALB/c mice were controlled by pregnant mare serum gonadtropin (PMSG) and human chorionic gonadotropin (HCG), and pregnant females were ovariectomized on the 4th day of pregnancy. The delay implantation state was maintained for 48 hr. After 0 to 18 hr of estrogen supply, a 1-hr pulse of3H-uridine or3H-leucine was injected intraperitoneally. The uterine tissues were processed for light and electron microscope radioautography, and analyzed at three distinct regions as follows: interimplantation site (IN), antimesometrial side (AI) and mesometrial side (MI) of the implantation site. The RNA and protein synthesis showed a peak at 6 hr after estrogen induction both in the AI stromal and epithelial cells, whereas in the cells of IN sites it increased only slightly. The presence of the blastocyst in the uterine lumen induced selective changes in the behavior of endometrial cells after the nidatory estradiol effect. The peak of RNA and protein synthesis seen in the AI cells reflected a commitment to restrict cell population during activation of the implantation window.
Published Version
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