RF-amide related peptide-3 neurons in the human hypothalamus. Distribution and efferent connections with gonadotropin-releasing hormone neurons

Abstract

Event Abstract Back to Event RF-amide related peptide-3 neurons in the human hypothalamus. Distribution and efferent connections with gonadotropin-releasing hormone neurons Csilla S. Molnár1, Zsolt Liposits1*, Eva Keller2, Imre Kalló1, Miklós Palkovits3 and Erik Hrabovszky1 1 Institute of Experimental Medicine Hungarian Academy of Sciences, Laboratory of Endocrine Neurobiology, Hungary 2 Semmelweis University, Department of Forensic Medicine, Hungary 3 Semmelweis University, Department of Anatomy, Histology and Embryology, Hungary The recently discovered RF-amide related peptides RFRP-1 and RFRP-3 are derived from a common peptide precursor and play a crucial role in the central regulation of reproduction. Studies of the RFRP neuronal system with immunocytochemistry are limited by the cross-reaction of commercially available antibodies with other members of the same peptide family, including prolactin releasing hormone and kisspeptins. In the present studies, a newly-developed mouse monoclonal antibody against RFRP-3 was used to study the distribution of RFRP-3 immunoreactive neurons and their fiber projections in the female human hypothalamus. While in mice, RFRP peptides are exclusively synthesized in the dorsomedial hypothalamic nucleus, we found many RFRP-3 immunoreactive somata along the wall of the third cerebral ventricle through the rostro-caudal extent of the human hypothalamus. The radioisotopic in situ hybridization detection of RFRP mRNA confirmed the presence of RFRP synthesizing neurons in the periventricular zone. Neuronal fibers immunoreactive to RFRP-3 densely innervated the medial hypothalamus and formed plexuses in the periventricular and infundibular nuclei. In rodents, RFRP neurons act on reproduction via innervating GnRH neurons. To address if this anatomical route also exists in the human, dual-label inmmunocytochemical experiments were carried out. RFRP-3 immunoreactive axons were found to form numerous contacts with the cell bodies and dendrites of GnRH neurons in the human hypothalamus. This study describes the distribution of RFRP synthesizing neurons in the human hypothalamus and their connection with GnRH cells. The neuroanatomical information will contribute to the understanding of central mechanisms whereby RFRP peptides regulate human fertility. This research was supported by the National Science Foundation of Hungary (OTKA K69127 and T73002). Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Homeostatic and neuroendocrine systems Citation: Molnár CS, Liposits Z, Keller E, Kalló I, Palkovits M and Hrabovszky E (2010). RF-amide related peptide-3 neurons in the human hypothalamus. Distribution and efferent connections with gonadotropin-releasing hormone neurons. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00104 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 23 Apr 2010; Published Online: 23 Apr 2010. * Correspondence: Zsolt Liposits, Institute of Experimental Medicine Hungarian Academy of Sciences, Laboratory of Endocrine Neurobiology, Budapest, Hungary, liposits@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Csilla S Molnár Zsolt Liposits Eva Keller Imre Kalló Miklós Palkovits Erik Hrabovszky Google Csilla S Molnár Zsolt Liposits Eva Keller Imre Kalló Miklós Palkovits Erik Hrabovszky Google Scholar Csilla S Molnár Zsolt Liposits Eva Keller Imre Kalló Miklós Palkovits Erik Hrabovszky PubMed Csilla S Molnár Zsolt Liposits Eva Keller Imre Kalló Miklós Palkovits Erik Hrabovszky Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.