Abstract
Combining transparent embedding with sectioning is likely to be the future direction for tissue clearing and 3-dimensional (3D) imaging. A newly published transparent embedding system, TESOS (Transparent Embedding Solvent System), ensures consistent submicron resolution imaging throughout the entire sample, and can be compatible with different microscopy systems. This method shows great potential in connectome mapping, and might be an optimal option for future 3D multiplex immunofluorescence and RNA insitu hybridization imaging. Additional efforts would be needed to innovate labeling, imaging, and data processing strategies to fully utilize the potential of transparent embedding systems in high-resolution imaging of large-scale samples.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call